PAM3CSK4 or PAM2CSK4 also elicited expression of genes in the later TLR2-dependent response component with delayed kinetics, evident by 4 hr post-infection but continuing to increase through 24 hr (Figure 4B, Figure 4—figure supplement 1B) As with Mtb infection, synthetic ligand-dependent expression of genes representative of both the early and late clusters was dependent upon TLR2 and the signaling adapter MYD88 (Figure 4C–D). Here, TLR2 is linked to infection.