To further investigate whether HAX1 serves as a downstream of HIF‐1α to maintain the survival ability and characteristics of glioma, we established LV3‐pGLV‐H1 + Puro plasmids with pcDNA‐HAX1 or control oligonucleotides (Lenti‐HAX1 and Lenti‐NC) to overexpress HAX1 protein expression. Here, HAX1 is linked to central nervous system cancer.