With the exception of ETV6-RUNX1 positive tumours the most frequent enriched motifs were the RAG heptamer and RAG nonamer, however in ETV6-RUNX1 the most common was the PRDM9 binding motif contained in 28% of breakpoints (P = 1 × 10−162) (Fig. 6b). Here, PRDM9 is linked to neoplasm.