As shown in Fig. 2G, yellow particles, indicating overlap between green and red fluorescence signals on merged images, could be observed around U-118 MG, U-87 MG and U-251 cells, while only red fluorescent particles were observed around HAs, which indicated that GBM cells loaded CD73 protein cargo into EVs (or exosomes) and secreted these vesicles. This evidence concerns the gene NT5E and glioblastoma.