To further explore whether GAS6-AS1 enhanced AML proliferation by regulation cell cycle progression in AML cells, flow cytometry was performed and demonstrated that HL-60 and U937 cells with sh-RNAs had a clear cell cycle arrest in the G1-S phase and the population of cells in the S phase was decreased (Fig. 3A-B). The gene discussed is GAS6; the disease is acute myeloid leukemia.