We transplanted sets of WT, Sharpincpdm (predisposed to cell death) or RIPK1D138N (resistant to cell death) B6 hearts exposed to 8 hours of CIS into CTLA4Ig-treated BALB/c recipients and quantified frequencies of splenic, donor-reactive IFN-γ– and TNF-producing CD8+ T cells 10 days later (Figure 3, A and B). Here, TNF is linked to in situ carcinoma.