We purified TEVs from the culture supernatants of B16 melanoma cells by centrifugation and phosphatidylserine receptor-conjugated beads (Figure 3D and E), which indeed expressed phosphatidylserine on the surface and bound to a chimeric fusion protein of the extracellular portion of CD300a with human IgG1 (Figure 3F). The gene discussed is CD300A; the disease is melanoma.