To further understand the consequences of HPSE regulation of β-catenin activation on HSV-1 infection, wild-type and Hpse-KO cells were primed with Wnt3a-CM for 12 or 24 h prior to infection with a KOS strain with an MOI of 0.1 for 24 h (Fig. 4C). This evidence concerns the gene HPSE and infection.