PML and infection: Mutation of 410lysine generated a series of successive effects during infection: it resulted in a remarkable decline in the UL44 SUMOylation level, disabled the U44 association and co-localization with PML, changed the UL44 nuclear distribution from ND10 foci to widespread diffusion, and more importantly, relieved HCMV from the ND10 restriction of host cells on viral replication and growth.