To verify whether RCN1 with a higher abundance in osteoblast‐CM might have an influence on RCN1 expression in NSCLC cells, NCI‐H1299 or NCI‐H460 cells were cultured in mixed medium (1640+CM, 1:1 mixture of RPMI‐1640 and CM from rat or human osteoblasts) and the protein and mRNA expression levels of RCN1 were measured using RT‐PCR and Western blotting assays, respectively. This evidence concerns the gene RCN1 and non-small cell lung carcinoma.