Additionally, after NCI‐H1299 cells transduced with shRCN1‐2 vector were injected in the proximal metaphysic of the tibia in nude mice for 8 weeks, the radiance (p/sec/cm2/sr) of tumour mass in shRCN1‐2 group was significantly lower than that in shcon group(Figure 4F) and the BV/TV in cortical bone in the tibia in shRCN1‐2 group was significantly higher than that in shcon group (Figure 4G), representing that RCN1 depletion in NCI‐H1299 cells reduced tumour growth accompanied with decreased bone resorption in cortical bone. This evidence concerns the gene RCN1 and neoplasm.