Studies in NASH model of wild-type and hepatocellular specific TGF-β receptor type II deficiency mice demonstrated that TGF-β signaling in hepatocytes promotes lipid accumulation by regulating lipid metabolism and enhancing cell death in hepatocytes that accumulate lipid, leading to the development of hepatic steatosis, inflammation, and fibrosis (183). This evidence concerns the gene TGFB1 and metabolic dysfunction-associated steatohepatitis.