RYR2 and squamous cell lung carcinoma: As shown in Figure 2A, the intersection genes with high mutations were TTN, TP53, MUC16, ZFHX4, FLG, LRP1B, SYNE1, RYR2, CSMD3, USH2A, MUC17, DNAH5, FAM135B, COL11A1, and RYR3. To further investigate whether these 15 commonly mutated genes were associated with TMB, lung squamous cell carcinoma patients from the TCGA cohort were classified into wild and mutated groups based on the 15 mutant gene statuses.