At the same time, we constructed the 2# shAR plasmid to knock down AR in HCC PLC/PRF/5 cells, and the results showed which could also increase the cells invasion and migration capacities, but the promotion effect was not as strong as the 1# shAR plasmid(Supplementary Figures 1A, B), so we chose the 1# shAR plasmid for further study. The gene discussed is AR; the disease is hepatocellular carcinoma.