To further evaluate the effect of LGALS8-AS1 on the metastatic ability of breast cancer cells, we transfected MDA-MB-231 and MCF-7 cells with two diverse short hairpin RNAs (shRNAs) against LGALS8-AS1 (sh-LGALS8-AS1-1 and sh-LGALS8-AS1-2) or an LGALS8-AS1-overexpressing plasmid (pcDNA-LGALS8-AS1). This evidence concerns the gene LGALS8 and breast cancer.