Weight gain in the PCOS animals increased significantly compared to the control group (p<0.001), while in the metformin- and trans-anethole (40 and 80)-treated animals it decreased significantly compared to the PCOS group (p<0.01-p<0.001).<h4>Conclusion</h4>These results showed that trans-anethole significantly decreased serum levels of insulin, DHEAS and blood lipids. Here, INS is linked to polycystic ovary syndrome.