The latency to enter the light chamber served as an anxiety index, and Nsf+/- mice exhibited a longer latency to enter the light box than Nsf+/+ mice (Nsf+/+ mice, 324.0 ± 54.3; Nsf+/- mice, 878.8 ± 205.8; Student’s t-test, *p = 0.02; Figure 4A). Here, NSF is linked to Anxiety.