Therefore, we compared ASC speck formation capacity (TOFIE) and IL-1β processing (inflammasome reconstitution assay) in HEK293Ts in response to agonists thought to use different activation pathways including pore formation/K+ efflux (nigericin), phagosome rupture (Fn infection and MSU stimulation), and ROS generation (H2O2). The gene discussed is IL1B; the disease is infection.