THP-1, THP-FAKi (THP-1 treated with FAK inhibitor) and THP-FAK+ (THP-1 overexpressing FAK) macrophages were infected with Mtb and culture supernatants were collected at 24 hours post-infection to quantify the levels of TNF-α, IL-1β and IL-10 using the solid-phase sandwich ELISA. This evidence concerns the gene TNF and infection.