AIF1 and hydrops fetalis: Using the ionized calcium-binding adaptor molecule-1 (Iba-1) as an immunofluorescence marker of microglia, we verified that HF diet increased the number of microglia in the HIP regions, including the cornu ammonis 1 (CA1), cornu ammonis 3 (CA3), and dentate gyrus (DG), while ESPs supplementation significantly decreased the number of microglia in the CA1, CA3, and DG (P < 0.05, Figures 2A, B).