In freshly isolated coronary arterial SMCs of ZDF rats at 8months after the development of hyperglycemia, BK channels had a rightward-shifted Ca2+ concentration-dependent curve, with increased EC50 for Ca2+ activation and decreased Ca2+ cooperativity, compared to those of Lean control rats (Lu et al., 2008). This evidence concerns the gene KCNMA1 and Hyperglycemia.