We then validated RNA-seq data by immunofluorescence and/or western blotting (WB) for some selected genes causing muscular dystrophies such as COL6A (Collagen Alpha 1, 2, and 3 type), EMD (Emerin), LMNA (Lamin A/C), DYSF (Dysferlin), and DAG1 (Dystroglycan 1), and another set of genes causing motor neuron diseases such as SMN, UBA1 (Ubiquitin-activating enzyme 1), DYNC1H1 (Dynein, cytoplasmic 1, heavy chain 1), SOD1, and C9orf72. Here, DAG1 is linked to muscular dystrophy.