TNF and migraine disorder: In addition, as shown in Figure 7, in model rats, NTG (NO donor) could cause vascular endothelial cell depolarization, cerebral vasodilation, and then caused aseptic inflammation, such as increased release of TNF-α and IL-6; at the same time, it activated the NO-cGMP-PKG pathway, the content of SP, CGRP and cGMP were increased, which led to migraine.