Astrocyte-derived EVs from brains and spinal cords of the SOD1-G93A ALS mouse model and the spinal cord of ALS patients with SOD1 mutant have been found to contain abundant misfolded and non-native disulfide-cross-linked aggregated SOD1 (Silverman et al., 2019). This evidence concerns the gene SOD1 and amyotrophic lateral sclerosis.