As shown in Figure 6A, H4/V1S:SV2/LAMP1-eCFP/mCherry-galectin-3 cells were infected with lentivirus carrying GBA1-knockout and control vectors, referred to as “GBA1-” and “WT.” After 3 days of infection, a portion of sibling cells from WT and GBA1- were harvested to evaluate the effect of GBA1 deletion on the level of GCase expression (Figures 6B,C) and sphingosine production (Figure 6D). Here, GBA1 is linked to infection.