N2a cells expressing Tau-CTF24 and Tau- FL and treated with Thioflavin S to measure aggregation SH-SY5Y cells expressing Tau-CTF24 and exposed to various seeds including heparin-induced assembled Tau, Tau- CTF24, or sarkosyl-insoluble pellets from human tauopathies. Here, MAPT is linked to tauopathy.