ZFAS1 and colorectal carcinoma: In this study, to further clarify the regulation pattern of IMP2 and ZFAS1 in CRC cell characteristics, we first analyzed the cell proliferation ability and colony formation capacity after interfering IMP2 expression, as detected by the MTT assay (Fig. 3b) and colony formation assays (CFA) (Fig. 3c) in HCT116 and SW620 CRC cells, respectively.