ZFAS1 and colorectal carcinoma: Thereafter, to investigate the potential roles of IMP2 in ZFAS1 expression and their associated m6A methylation levels, we, for the first time, performed tissue microarray (TMA), RNA in situ hybridization (ISH), and immunohistochemistry (IHC) assays to detect the expressions of IMP2, m6A, and ZFAS1 in our relatively large samples of paired CRC tissues and matched adjacent-tumor controls (n = 144) (Fig. 1h).