ZFAS1 and colorectal carcinoma: To verify the reliability of the binding sites of IMP2 and ZFAS1, ISH and immunofluorescence (IF) assay for colocalization were performed and the consistent distributions of ZFAS1 and IMP2 expressions were confirmed, with the main locus in the cytoplasm and some parts in the nucleus of HCT116 and SW620 CRC cells (Fig. 2j).