To further elucidate the critical domain of the IMP2 protein involved in stabilizing ZFAS1 expression and CRC cell fate, the RNAfold (http://rna.tbi.univie.ac.at/cgi-bin/RNAWebSuite/RNAfold.cgi) webserver was explored to predict the secondary structure of ZFAS1 (Additional file 2: Fig. S2c). The gene discussed is IGF2BP2; the disease is colorectal carcinoma.