IGF2BP2 and colorectal carcinoma: More importantly, RNA stability assays further identified that the half-life of ZFAS1 RNA was reduced by IMP2 knockdown or by actinomycin D treatment at different time points (0, 2, 4, and 6 h) in HCT116 and SW620 CRC cells compared with the half-life in cells treated with an empty vector (negative control group) (HCT116: shNC t1/2 = 15.40; shIMP2 t1/2 = 5.06; SW620: shNC t1/2 = 11.36; shIMP2 t1/2 = 2.16, Fig. 2d).