In this study, whether transfected with miR-16-5p mimcis or si-ANLN or both cotransfection compared with NC groups, we found that cell proliferation slowed down and cell migration and invasion were inhibited in MCF-7 and MDA-MB-231 cells (Figs. 2l-m, 3a-d), indicating that miR-16-5p suppressed cell proliferation, migration and invasion by downregulating ANLN in BC cells. Here, ANLN is linked to breast cancer.