To decipher the contribution of bacterial and fungal microbiota to the enhanced susceptibility of Dok3−/− mice to colitis, we fed WT and Dok3−/− mice ad libitum with either a broad-spectrum antibiotics cocktail (consisting of vancomycin, ampicillin, neomycin, and metronidazole) or antifungal fluconazole to suppress the growth of bacteria or fungi species in their gut, respectively. Here, DOK3 is linked to colitis.