TFRC and cervical carcinoma: By western blotting, we found that Cdc25A overexpression alone increased p62 and FTH1 levels but decreased the LC3-II/LC-I ratio and TfR1 level in cervical cancer cells, while PKM2-S37D overexpression showed the opposite effects (Fig. 5A), suggesting that autophagy is suppressed by Cdc25A but enhanced by PKM2 Ser37 phosphorylation.