Additional experiments were designed to further test whether d-lactate production depends upon the expression of GLO1. Murine lung cancer cells (3553T3) expressing a guide RNA targeting Glo1 (sgGlo1) to knock out expression of this gene, and an isogenic line in which Glo1 was re-expressed (355T3 sgGlo1 pLHCX Glo1 )[23] were incubated in the presence of 5 mM MG for 4h. This evidence concerns the gene GLO1 and lung carcinoma.