Moreover, depleting creatine levels in thermogenic adipocytes of mice by deleting the rate-limiting enzyme of creatine biosynthesis, glycine amidinotransferase (GATM), impairs energy expenditure due to reduced thermogenesis and causes diet-induced obesity [67, 68]. The existence of this cycle in BAT supports the concept that increasing mitochondrial ATP synthesis can be used as an approach to promote energy dissipation. This evidence concerns the gene GATM and obesity disorder.