To validate the functional significance of aberrant nuclear expression of STEAP3 as observed in HCC tissues, we first examined its expression level in HCC cell lines and immortalized hepatocyte MIHA (Fig. 2A), and accordingly established HCC cell lines in which STEAP3 was stably upregulated (PLC/PRF/5-STEAP3) or knockdown (Hep3B-ShSTEAP3 and SNU449-ShSTEAP3) (Fig. 2B). The gene discussed is STEAP3; the disease is hepatocellular carcinoma.