In this study, we confirmed that SNS-032 inhibited migration and invasion of ESCC cells via transcriptional inhibition of MMP-1, which was based on the following results: (1) Both the mRNA and protein levels of MMP-1 in ESCC cells were downregulated upon treatment with SNS-032 in a concentration-dependent manner. The gene discussed is MMP1; the disease is esophageal squamous cell carcinoma.