SHANK3 and Cognitive impairment: Given that ASD-associated behaviors and co-morbidities such as hyperactivity, anxiety, and cognitive impairment have been observed in Shank2 KO, Shank3 KO, and prenatal zinc-deficient mice [35, 43], in the first set of experiments, we tested the locomotor performance and anxiety-related behaviors in the open field (OF) test [45] (Fig. 5).