We found that the formation of the p26 cleavage fragment of CASP11 was reduced in Casp6−/− BMDMs infected with either E. coli or C. rodentium for 10 and 20 h (Fig. 3, C and D), suggesting that CASP6 is involved in the processing of CASP11 for its activation during gram-negative bacterial infection. Here, SCAF11 is linked to gram-negative bacterial infections.