To investigate whether CASP6 also has a role in the regulation of the CASP11-NLRP3 inflammasome during gram-negative bacterial infections, we infected bone marrow-derived macrophages (BMDMs) isolated from WT and Casp6−/− mice with the enteric bacteria Escherichia coli or Citrobacter rodentium. This evidence concerns the gene NLRP3 and gram-negative bacterial infections.