Actually, in our system, exposure to CPZ prompted nuclear translocation of cleaved ATF6-α in both anchorage-dependent GBM cells and neurospheres, as indicated by western blotting determinations on separate cytoplasmic and nuclear protein fractions (blots and histograms) and representative immunofluorescence images as well (Fig. 2 A and B). This evidence concerns the gene ATF6 and glioblastoma.