In subsequent analysis of the cellular response to CBK79, we found that administration of CBK79 to human osteosarcoma (HOS) cells expressing green fluorescent protein (GFP)-tagged MAP1LC3B/LC3B (microtubule-associated protein 1 light chain 3 beta) caused the formation of cytosolic GFP-LC3B foci, which is indicative for an effect on autophagy (Figure 3A). Here, MAP1LC3B is linked to osteosarcoma.