Third, to test the cDC-generating capacity of CD11blo and CD11b- pre-cDC populations in an inflammatory setting, we sorted them (as indicated in Fig 1A, gates 4), as well as CD16/32+ (CD11c+ MHC-II- CD11bhigh) cells (Fig. 1E) and Ly6C+ monocytes (Fig. S1A; used as a control), from the lungs of CD45.1 mice at 1 day post-infection (dpi) with IAV. This evidence concerns the gene ITGAM and infection.