The results showed that IR could significantly increase the accumulation of NIK protein, a master kinase of the noncanonical NF‐κB signaling pathway, in ESCC TE‐1 and ECA‐109 cells and in SCC‐9 and SiHa squamous carcinoma cells (Fig. 5A,B, Fig. S4C). The gene discussed is NFKB1; the disease is esophageal squamous cell carcinoma.