The KEGG pathway enrichment analysis showed that target genes were enriched in the pathways including pathways in cancer, cell cycle pathway, PI3K–Akt signaling pathway, MAPK signaling pathway, Ras signaling pathways, and Hippo signaling pathways, which may contribute to ESCC cell proliferation and tumorigenesis (Figure 3B). This evidence concerns the gene AKT1 and esophageal squamous cell carcinoma.