To verify the biological roles of CDCA7 in ESCC, we first analyzed the mRNA and protein expression levels in immortal embryonic esophageal epithelium cell lines NE3 and HET-1A and ESCC cell lines including KYSE150, KYSE180, KYSE450, and TE-1 via quantitative real-time PCR (q-RTPCR) and Western blot (Figure S3). Here, CDCA7 is linked to esophageal squamous cell carcinoma.