After exclusion (Supplementary Table 2), 17 cases of melanoma were positive for IGF2BP3 and had a high positive rate, and 8 cases in normal skin tissues (50%) were weakly positive for IGF2BP3 (Figure 11A).The difference between the positive rate of IGF2BP3 in melanoma and normal skin was seen(Figure 11B).7 cases of primary melanoma (87.5%) were positive for SPRR1B, and 2 cases in metastatic melanoma (25%) were very low weakly positive for SPRR1B (Figure 11C).The difference between the positive rate of SPRR1B in Primary melanoma and metastatic melanoma was seen in Figure 11D. This evidence concerns the gene SPRR1B and metastatic melanoma.