Since CXCR4 has been implicated in homing of leukemia cells to their niche (27, 28), we tested the effect of plerixafor (AMD3100) in the colonization of AMD3100-treated B-ALL cells to the 3D structures and found that it can only partially prevent B-ALL cell spheroid colonizing, with a 4.6-fold decrease (Figure 2B). This evidence concerns the gene CXCR4 and precursor B-cell acute lymphoblastic leukemia.