We found that the immunosuppression drug Cyclosporin A (CsA), which targets the positive-strand RNA virus-interacting protein Cyclophilin A (PPIA), inhibited SARS-CoV-2 (WT) infection with an IC50 (the half maximal inhibitory concentration) value of 1.52 μM and a CC50 (the half maximal cytotoxic concentration) value of > 30 μM in A549ACE2 (a selective index (SI) > 19.74). This evidence concerns the gene PPIA and infection.