Although all H3F3AK27M and EZHIP cases clustered with other diffuse midline glioma K27M tumors, the HIST2H3CK27M case (ICR-B184) was most similar to ICR-B118 and other MYCN-amplified glioblastoma in the t-statistic–based stochastic neighbor embedding projection of the methylation array data (Fig. 1B). Here, MYCN is linked to diffuse midline glioma.