Among 19,073 known genes sequenced, variant analysis firstly focused on three genes associated with FHH, including CASR, GNA11, and AP2S1. The further variant analysis was carried out on MEN1, RET, CDKN1B, GCM2, CDC73, and other genes associated with PHPT induced hypercalcemia. This evidence concerns the gene AP2S1 and familial hypocalciuric hypercalcemia.