The upregulated miRNAs mostly targeted signaling pathways such as PI3K-Akt, Ras, cAMP, chemokine and cytokine-cytokine receptor interaction and endocytosis, while the downregulated miRNAs mostly targeted signaling pathways such as MAPK, Ras, Rap1, Wnt and pathways in cancer (Fig 4A and 4B). This evidence concerns the gene AKT1 and cancer.