It was discovered in the gene detection in this study that the mRNA levels of MUC2, MUC5A and pathway genes TLR4 and JNK in the model group were raised, while those in the treatment group were evidently decreased, implying that the pathway genes may be activated during the pathogenesis of otitis media, which further indicates the further progression of the disease. The gene discussed is MAPK8; the disease is otitis media.