The results show that Tfh cells and Tregs have a higher proportion in patients with high tumor stage than in stage I, but decreased level trend of M0, M1 macrophages infiltration, compared with that of stage I tumors (Figures 8D,E), suggesting that with the progression of BRCA1/2-mutant BC, the proportions of infiltrating immune cells showed an increased trend, especially Tfh and Tregs. The gene discussed is BRCA1; the disease is breast cancer.