In the discovery cohort, among the top 35 mutated genes, five genes (MGA, PBRM1, PTPRD, NOTCH1, ZFHX3) were found to be correlated to ICI outcome in NSCLC patients, while only three genes (MGA, PTPRD, ZFHX3) were significantly associated with patients’ survival in the validation cohorts. This evidence concerns the gene MGA and non-small cell lung carcinoma.