In the discovery cohort, among the top 35 mutated genes, five genes (MGA, PBRM1, PTPRD, NOTCH1, ZFHX3) were found to be correlated to ICI outcome in NSCLC patients, while only three genes (MGA, PTPRD, ZFHX3) were significantly associated with patients’ survival in the validation cohorts. The gene discussed is NOTCH1; the disease is non-small cell lung carcinoma.