The successful up-regulation of ITGB1 was verified through RT-PCR and Western blot methods (Figures 8A,B), and the effects of TGF-β conditioned MSCs on the migration, invasion and adhesion of leukemia cells in the control group, the EV group, and the LV-ITGB1 group were detected, AMD3100 was used to interfere with the interaction between leukemia cells and TGF-β conditioned MSCs. This evidence concerns the gene TGFB1 and leukemia.