Western blot results revealed that the ITGA5 and ITGB1 in leukemia cells in the co-culture system of TGF-β conditioned MSCs group were significantly higher than those in the ALL cells culture alone group (P < 0.05; Figures 6A,B), but had no obvious difference compared with the group of co-culture system of MSCs and ALL cells, aside from ITGA5 in leukemia cells in the co-culture system of MSCs and RS4;11 cells (Figures 6A,B). This evidence concerns the gene ITGB1 and acute lymphoblastic leukemia.